Haematology & Clinical Cytopathology


The policy of the department is to:
Develop and apply state of the art investigative facilities for the comprehensive diagnosis of haematologic diseases
Participate in decision making on management of patients with haematological disorders
Ensure quality at all times and levels
Deliver service with compassion and understanding keeping different cost structures for different strata of society

About the Department

The objectives of the haematology diagnostic service in this hospital are to provide accurate and reliable test results in acceptable turn-around time ensuring continuous quality. There is a constant effort to keep pace with advances in technology and laboratory investigations. The department has made rapid strides in developing investigative facilities for groups of disorders such as hemopoietic malignancies, coagulation disorders, thalassemias & hemoglobinopathies, auto-immune disorders etc and utilises state-of-the-art technology (including flow cytometry).

Some of the functions of the department are as follows:

  • Screen for possible abnormality - pre-operative blood tests, ante-natal screening, health check-up
  • Diagnose or exclude blood disease or to help establish a specific diagnosis
  • Monitor treatment and provide appropriate supportive care
  • Provide haematological tests in an emergency 24-hours a day
  • Provide specialist investigations necessary for diagnosis & / or monitoring of blood diseases
  • Evaluate new methods and equipment
  • Provide laboratory facilities for haematological research

Services provided

Routine investigations ( Bayers Advia 120 and 60 Haematology Analysers, ACL 7000)

Complete blood counts

Hb, Haematocrit RBC, MCV, MCH, MCHC, RDW, Platelet count , TLC, MPV, differential & reticulocyte counts (Bayers Advia 120 Haematology Analyser) 

Absolute eosinophil, count (AEC) , Erythrocyte sedimentation rate (ESR), peripheral blood film examination for morphology and parasites

Bone marrow examination

More than 100 bone marrow specimens (aspirates, imprints and biopsies) are received annually. Several rare disorders are diagnosed each year. Immunohistochemistry is routinely preformed on biopsy specimens.

Hemostatic disorders

 (Instrumentation Laboratory ACL 7000 fully automated coagulometer)

Haemorrhagic disorders

Prothrombin time (PT)*, Activated partial thromboplastin time (APTT), Thrombin time (TT), assays of factor VIII and IX, Platelet count, Fibrinogen estimation, Fibrinogen degradation products (D-dimer), Bleeding Time (BT), Clot retraction test, Clot solubility test (factor-XIII), APTT mixing experiment, PT mixing experiment. (*=PT is reported in INR)


For antiphospholipid syndrome and lupus anticoagulants: APTT with lupus sensitive reagent, APTT - dependent inhibitor screen, Dilute Russell Viper Venom Time (dRVVT), Kaolin clotting time (KCT), Anti-cardiolipin antibodies (ANA), Anti-ds-DNA antibodies

ProC global screening test; Protein C, Protein S & Antithrombin (clot based functional assays)

Hemolytic anemias

Screening / testing for beta-thalassemia & hemoglobinopathies by HPLC (BioRad Variant I) and hemoglobin electrophoresis at alkaline & acid pH (Beckman Coulter Appraise system): Some of the interesting patterns are Hb DIran, Hb QIndia, Hb E, Hb C, Hb J etc.G-6-PD screening

Osmotic fragility test, Acidified serum (Ham) test, Autohemolysis test, Heat instability test for unstable hemoglobin, Heinz bodies, Haemoglobin F cytochemistry (Kleihauer test), Isopropanol stability test, Hemosiderin in urine, Serum haptoglobin estimation, Sucrose lysis test, Methemoglobin estimation, sickling test for Hb S

Leukemias & lymphomas

Detailed work-up by CBC, bone marrow examination, cytochemistry and flow cytometry on acute and chronic leukemias, myelodysplastic syndromes (MDS), myeloproliferative disorders (MPD) and lymphoproliferative disorders (LPD)

Flow cytometry


 (Beckman Coulter Epics XL flow cytometer)

Leukemia and lymphoma immunophenotyping (Acute leukemia panel, CLL panel, Lymphoma panel)

HIV panel (CD3, CD4 & CD8 counts)

Enumeration of B-, T- & NK cells

PNH panel (CD55 & CD59)

Other special investigations

Serum protein electrophoresis (SPE), Immunofixation electrophoresis ( IFE ) Urinary protein electrophoresis (concentration by Minicon filter).

Collagen disease markers: ENA plus (screening), ds-DNA, p-ANCA, c-ANCA by ELISA

Cervical & Vaginal Cytology


Done for local Pathology-Trichomonas vaginalis, candida, Gardnella vaginalis,Cervical & Vaginal Cancer Screening,

Sputum Cytology, Bronchial Washings, Broncho- Alveolar Lavage, Liver Abscess Aspirate, Urine Cytology

Done for Local Pathology and Cancer Screening


Done for Local Pathology and Cancer Screening

Clinical & HistoPathology
  • Urine: Routine and microscopic examination
  • Stool: Routine and microscopic examination, Occult Blood Test
  • Special: Urobilinogen, porphobilinogen, hemoglobin, myoglobin, chyle
  • Body fluids: Ascitic fluid, Cerebrospinal fluid, Gastric aspirate for neutrophils Hemosiderin in urine, Pericardial fluid, Pleural fluid, Synovial fluid
  • Biopsies: Specimens removed during surgery are sent for Microscopic examination for Local Pathology and Cancer Screening

Quality assurance
Quality assurance is a major concern of the department. It covers all aspects of laboratory functioning i.e. pre-analytical, analytical and post-analytical; turn around time; maintenance of equipment; selection of tests & reagents; standardization & validation of tests before they are introduced; and training of personnel.

Our Consultants

Phone No
Dr. Manorama Bhargava
Dr. Rashmi Vohra
MBBS,(MD Pathology)Diploma in Pathology & Bacteriology(DPB)
E-mail :- pathlab@cityhospitalindia.in